Pilin regions that select for the small RNA phages in <i>Pseudomonas aeruginosa</i> type IV pilus.

ABSTRACT: Type IV pili (TFPs) serve as the primary receptor for phages in Pseudomonas aeruginosa (PA), playing a key role in phage selection and determining the phage host range. We previously demonstrated that a fiersphage Pepevirus rubrum (PP7) selectively infects PA strains with group II (G2) TFP pilin. In this study, we expanded on this by profiling the host range of another fiersphage, Pepevirus spumicola (LeviOr01), which was able to infect several PA strains including PMM23. Analysis of 47 in-house PA strains showed that PMM23, along with nine other LeviOr01-susceptible strains and four resistant strains, possessed group I (G1) TFP pilin. This suggests that LeviOr01 targets specific regions of G1 pilin for phage-receptor interaction. Comparative sequence analysis revealed

SUBMITTER: Bae H-W 

PROVIDER: S-EPMC11998500 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature