Integrating CORUM and co-fractionation mass spectrometry to create enhanced benchmarks for protein complex predictions.

ABSTRACT: Co-fractionation mass spectrometry (CFMS) enables the discovery of protein complexes and the systems-level analysis of multimer dynamics that facilitate responses to environmental and developmental conditions. A major challenge in CFMS data analysis, and omics approaches in general, is the development of reliable benchmarks for accurate evaluation of prediction methods. CORUM is commonly used as a source of benchmark complexes for protein complex composition predictions; however, its assumption of fully assembled subunit pools often conflicts with size exclusion chromatography (SEC) and interaction predictions from CFMS experiments. To address this, we developed an integrative analysis method that leverages cross-kingdom evolutionary conservation among specific CORUM complexes and high-res

SUBMITTER: Yang P 

PROVIDER: S-EPMC11998666 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature