ViraLite: An Ultracompact HIV Viral Load Self-Testing System with Internal Quality Control.

ABSTRACT: The availability of effective antiretroviral therapy has made HIV manageable, provided patients have consistent access to routine viral load (VL) testing. Nonetheless, access to frequent VL testing remains limited. There is a need for accessible, user-friendly testing systems that allow people living with HIV (PLHIV) to monitor their VL more frequently and empower self-management. Here, we developed ViraLite, a sample-to-answer, compact, accessible, and battery-powered system for HIV viral load monitoring. The system is built upon a probe-based RT-LAMP assay that allows for multiplexed detection and quantification. An internal quality control targeting the RNase P was incorporated to enhance the reliability of the results. A software-reconfigurable real-time sensing system empowered by mac

SUBMITTER: Politza AJ 

PROVIDER: S-EPMC11998842 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature