Mapping the antibody response to Lassa virus vaccination of non-human primates.

ABSTRACT:

Background

Lassa fever, caused by Lassa virus, is a severe disease, endemic in Western Africa, for which no vaccines or therapeutics are yet approved. Understanding the immune responses elicited by candidate vaccines is key for approval, including characterisation of antibody epitopes recognised and capacity for neutralisation.

Methods

Here we used negative-stain electron microscopy polyclonal antibody epitope mapping (EMPEM), in-vitro pseudovirus neutralisation assays, and biophysical antibody competition assays to uncover components of polyclonal antibody responses elicited in nonhuman primates 26 days after receipt of a single immunisation with a fully protective, recombinant, replication-competent vesicular stomatitis virus-based vaccine bearing the Lassa virus glycoprot

SUBMITTER: Enriquez AS 

PROVIDER: S-EPMC11999075 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature