Multicentric validation of the proof of concept for utility of Trueprep extracted DNA in line probe assay testing.

ABSTRACT: In India's National Tuberculosis Elimination Program (NTEP), diagnosing tuberculosis (TB) and Drug-Resistant TB (DR-TB) involves collecting two sputum specimens from presumptive TB patients. Mycobacterium tuberculosis (MTB) is detected using the Truenat MTB - RIF assay at peripheral sites, with Trueprep for DNA extraction. In the case of a MTB positive, the second specimen is sent to a linked reference laboratory for Line Probe Assay (LPA) testing, requiring DNA extraction again. This study aimed to validate Trueprep-extracted DNA for First Line (FL) and Second Line (SL) LPA testing to assess real-time programmatic field conditions and DNA transportation across diverse Indian geographies. Four hundred MTB-positive Trueprep extracted DNA with ≥104 cfu/ml value from the first specimen, accom

SUBMITTER: Rajendran P 

PROVIDER: S-EPMC11999121 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature