A novel <i>in vitro</i> system for simultaneous infections with hepatitis B, C, D and E viruses.

ABSTRACT:

Background & aims

The liver, and more precisely hepatocytes, can be infected by several hepatotropic viruses, including HBV, HDV, HCV and HEV, with chronic infection leading to end-stage liver diseases. Since no in vitro model allowing multi-infections with the four viruses is reported, limited data are available on their interplay as well as on the potential cross-reactivity of antivirals in multi-infection cases. The aim of our study was to set up such a model.

Methods

HuH7.5-NTCP cells were cultured with 2% DMSO (dimethyl sulfoxide) for 1 week to allow partial differentiation into hepatocytes (dHuH7.5-NTCP) before infection with the different viruses and treatment with known antiviral molecules.

Results

We observed increased expression of liver specific tran

SUBMITTER: Fouille R 

PROVIDER: S-EPMC11999259 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature