Contrast-Enhanced Ultrasound to Assess Kidney Quality During <i>Ex Situ</i> Normothermic Machine Perfusion.

ABSTRACT: Normothermic machine perfusion (NMP) provides opportunity for viability assessment of donated kidneys. Diminished microvascular perfusion, despite adequate total blood flow, is a key pathophysiology in ischaemia-mediated acute kidney injury. Contrast-enhanced ultrasound (CEUS) could allow objective assessment of microvascular perfusion during renal NMP. Blood-based NMP was performed on porcine kidneys (circulatory death model) and human kidneys declined for transplant (preclinical). CEUS was performed with a contrast bolus into the NMP circuit arterial limb. Microvascular perfusion quality was quantified and z-score normalisation allowed combination of metrics and regions into an overall "CEUS-score." In porcine kidneys, inferior microvascular perfusion of cortex and medulla correlated wit

SUBMITTER: Tingle SJ 

PROVIDER: S-EPMC11999844 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature