Heme and iron limitation in a GI-tract foundation species leads to a reshuffling of the metalloproteome and a shift toward manganese usage.

ABSTRACT: The metal-binding complement of the cellular proteome (the metalloproteome) depends on metal availability in the cellular environment and drives cellular metabolism. Bacteroides thetaiotaomicron (Bacteroides theta) is a foundational species in the anaerobic gut microbiome and a heme auxotroph, though little is known about why it requires heme. We hypothesized that B. theta would overproduce heme-binding proteins in response to limitations in non-heme iron, and reciprocally, activate non-heme iron pathways when heme was growth limiting. Here we showed that heme and/or non-heme iron scarcity triggers a more holistic reorganization of its metallome and metalloproteome. Under non-heme iron limitation induced by an Fe(II)-specific chelator, manganese supplementation restore

SUBMITTER: Rodrigues da Silva R 

PROVIDER: S-EPMC12000045 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature