Keep the Hospital Clean: Diagnostic Performance of Ten Different Molecular and Culture-Based Methods to Detect Candidozyma (Candida) auris.

ABSTRACT:

Rationale

Candidozyma auris (formerly Candida auris) is a globally emerging potentially multi-drug resistant human pathogenic yeast. To detect C. auris we aimed to compare different culture-, and molecular-based methods.

Methods

Rectal swabs routinely collected in clinical care were spiked with different concentrations of C. auris. Co-infection/colonization was mimicked by spiking part of these samples with other pathogenic Candida species. Spiked materials were cultured at 37 or 42 °C using CHROMagar Candida and CHROMagar Candida Plus plates. In parallel, samples were incubated in a dulcitol salt enrichment broth. Additionally, we compared seven in-house and commercial molecular tests on the direct material and from the broth one day after inoculation.

Results

Cultur

SUBMITTER: Korsten K 

PROVIDER: S-EPMC12000201 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature