Hypothermic machine perfusion prevents hyperacute graft loss in pig-to-primate kidney xenotransplantation after 5-hours of cold Ischemia.

ABSTRACT:

Background

Xenotransplantation (XTx) is a promising strategy to address the organ shortage. Clinical application will likely require off-site procurement from designated pathogen-free (DPF) facilities, introducing unavoidable cold ischemic time (CIT). The impact of CIT and organ preservation method on graft function in XTx remains unclear.

Methods

We evaluated eight cases of pig-to-baboon kidney xenotransplantation performed after five hours of CIT, comparing static cold storage (SCS) to hypothermic machine perfusion (HMP) preservation. Outcomes were assessed relative to six additional pig-to-baboon transplants performed with minimal CIT.

Results

All grafts preserved with SCS experience hyperacute rejection within 90 min of reperfusion, even in recipients with low lev

SUBMITTER: Hisadome Y 

PROVIDER: S-EPMC12000405 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature