High-sensitivity real-time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures.

ABSTRACT: Erlenmeyer shake flasks are widely used during the first steps of bioprocess development. Despite their broad application in academia and industry, shake flasks usually lack standardized and user-friendly online monitoring techniques. In this work, the pH and Respiratory Activity MOnitoring System (pH-RAMOS) for the non-invasive online measurement of the oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and pH in up to eight parallel shake flasks under sterile conditions is presented. The OTR and CTR are quasi-continuously measured in the headspace of the shake flasks using dedicated oxygen and carbon dioxide sensors, enabling precise respiratory quotient (RQ) evaluation. Self-adhesive pH sensor spots are used for the high-frequent real-time pH monitoring of the culture. Thes

SUBMITTER: Sarikaya B 

PROVIDER: S-EPMC12000638 | biostudies-literature | 2025 Mar-Apr

REPOSITORIES: biostudies-literature