<i>PEX3</i> gene knockout influences recombinant xylanase expression by <i>Komagataella phaffii</i>.

ABSTRACT: Komagataella phaffii is a methylotrophic yeast harboring a tightly regulated alcohol oxidase promoter (P _AOX1 ), which is now widely used for recombinant protein production. During P _AOX1 expression phase by methanol induction, a methanol metabolism organelle peroxisome enlarged and occupied 80 % of K. phaffii cell through peroxins functions of matrix protein import and organelle division. Using a K. phaffii expressing xylanase in this study, each of all 23 PEX genes of K. phaffii, encoding peroxin, was knockout to influence the peroxisome size, leading to changes of K. phaffii physiological status and recombinant xylanase expression. It was observed that PEX3 knockout reduced peroxisome size

SUBMITTER: Zhou Z 

PROVIDER: S-EPMC12000700 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature