Investigating the interplay between RNA structural dynamics and RNA chemical probing experiments.

ABSTRACT: Small molecule chemical probes that covalently bond atoms of flexible nucleotides are widely employed in RNA structure determination. Atomistic molecular dynamic (MD) simulations recently suggested that RNA-probe binding can be cooperative, leading to measured reactivities that differ from expected trends as probe concentrations are varied. Here, we use selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE), dimethyl sulfate (DMS) chemical probing, and nuclear magnetic resonance (NMR) spectroscopy to explore the relationship between RNA structural dynamics and chemical probe reactivity. Our NMR chemical exchange experiments revealed that SHAPE-reactive base-paired nucleotides exhibit high imino proton exchange rates. Additionally, we find that as the concentration of a probe

SUBMITTER: Arnold EB 

PROVIDER: S-EPMC12000872 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature