NicOPURE: nickless RNA circularization and one-step purification with engineered group II introns and cyclizing UTRs.

ABSTRACT: Circular RNAs (circRNAs) have gained significant attention in recent years due to their diverse biological functions and potential as novel therapeutic modality. Two general mechanisms for generating circRNAs involve the utilization of group 1 and group 2 introns, which are self-splicing ribozymes found in many organisms. Although group 1 intron has been demonstrated to be highly effective in circularization, the reaction requires high temperature plus cofactors such as GTP. Consequently, undesired byproducts such as nicked RNA were generated, which requires complex purification steps before downstream applications. In this study, we have strategically designed structural elements and incorporated sequence features to enhance the efficacy of RNA circularization by group 2 introns. This inn

SUBMITTER: Gao X 

PROVIDER: S-EPMC12000875 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature