Systematic genetic perturbation reveals principles underpinning robustness of the epigenetic regulatory network.

ABSTRACT: The molecular control of epigenetic information relies on hundreds of proteins of diverse function, which cooperate in defining chromatin structure and DNA methylation landscapes. While many individual pathways have been characterized, how different classes of epigenetic regulators interact to build a resilient epigenetic regulatory network (ERN) remains poorly understood. Here, we show that most individual regulators are dispensable for somatic cell fitness, and that robustness emerges from multiple layers of functional cooperation and degeneracy among network components. By disrupting 200 epigenetic regulator genes, individually or in combination, we generated network-wide maps of functional interactions for representative regulators. We found that paralogues represent only a first layer

SUBMITTER: Wilson TS 

PROVIDER: S-EPMC12000879 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature