High Agreement Across Laboratories Between Different Alpha-Synuclein Seed Amplification Protocols.

ABSTRACT:

Background

Seed amplification assays (SAA) detect alpha-synuclein (aSYN) pathology in patient biomatrices such as cerebrospinal fluid (CSF)-potentially even before clinical manifestations. As CSF-based SAA are approaching broader use in clinical trials and research, ensuring that different laboratories obtain the same results becomes increasingly important.

Methods

In this cross-laboratory, cross-aSYN-recombinant substrate and cross-protocol round-robin test, we compared SAA results from a common set of 38 CSF samples measured independently in four research laboratories of the German Center for Neurodegenerative diseases. Three laboratories (A-C) used an assay protocol adapted from Parchi's group at ISNB (Bologna, Italy); laboratory D used an assay protocol adapted from Ampr

SUBMITTER: Brauer S 

PROVIDER: S-EPMC12000918 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature