Succinate receptor 1 signaling mutually depends on subcellular localization and cellular metabolism.

ABSTRACT: Succinate is a pivotal tricarboxylic acid cycle metabolite but also specifically activates the G_i- and G_q-coupled succinate receptor 1 (SUCNR1). Contradictory roles of succinate and succinate-SUCNR1 signaling include reports about its anti- or pro-inflammatory effects. The link between cellular metabolism and localization-dependent SUCNR1 signaling qualifies as a potential cause for the reported conflicts. To systematically address this connection, we used a diverse set of methods, including several bioluminescence resonance energy transfer-based biosensors, dynamic mass redistribution measurements, second messenger and kinase phosphorylation assays, calcium imaging, and metabolic analyses. Different cellular metabolic states were mimicked using glucose (Glc) or gluta

SUBMITTER: Liebing AD 

PROVIDER: S-EPMC12001207 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature