MK5 Regulates Microglial Activation and Neuroinflammation in Experimental Stroke Models.

ABSTRACT:

Objective

Microglial activation plays a crucial role in neuroinflammation following ischemic stroke. This study was conducted to investigate the role and potential mechanisms of MK5 within microglial cells in the inflammatory response following ischemic stroke in mice in vivo and in vitro.

Methods

Microglia-specific conditional MK5 knockout (MK5 cKO) mice and their control mice (MK5^f/f) were subjected to middle cerebral artery occlusion (MCAO). BV2 cells (a mouse microglial cell line) were transfected with small interfering RNA (siRNA) to knock down MK5 levels and subsequently exposed to oxygen-glucose deprivation/reperfusion (OGD/R) to simulate ischemic conditions in vitro. Following MCAO, behavioral tests and infarct volume measurements were conducted. Levels of

SUBMITTER: Wang X 

PROVIDER: S-EPMC12001269 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature