Short- and mid-term temporal variability of the human urinary microbiota: a prospective observational cohort study.

ABSTRACT:

Background

Understanding the temporal variability of the microbiome is critical for translating associations of the microbiome with health and disease into clinical practice. The aim of this study is to assess the extent of temporal variability of the human urinary microbiota. A pair of urine samples were collected from study participants at 3-40-month interval. DNA was extracted and the bacterial V4 hypervariable region of the 16S rRNA gene was sequenced on the Illumina MiSeq platform. The alpha diversity of paired samples was analyzed using Chao1 and Shannon indices and PERMANOVA was used to test the factors influencing beta diversity.

Results

A total of 63 participants (43 men and 20 women with a mean age of 63.0 and 57.1 years, respectively) were included in the final an

SUBMITTER: Tlaskal V 

PROVIDER: S-EPMC12001408 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature