Design and verification of a 25 K multiple-SNP liquid-capture chip by target sequencing for dairy goat.

ABSTRACT:

Background

In the genetic breeding research of dairy goats, traditional genotyping methods have limitations, and existing goat chips have shortcomings in functional loci and other aspects, which cannot meet the precise genetic analysis needs of dairy goats. Genotyping by Target Sequencing (GBTS) in the new generation of sequencing technology provides the possibility to solve these problems.

Results

A large number of candidate SNP sites related to important economic traits in dairy goats were identified through various analysis and screening methods. The chip ultimately retained 27,396 SNP sites for probe design, which can detect 46,459 SNPs. The site distribution is uniform, and the sequencing data efficiency, base quality, alignment rate, and other indicators are good. The

SUBMITTER: Zhao J 

PROVIDER: S-EPMC12001634 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature