Rapid isolation of extracellular vesicles from stem cell conditioned medium using osmosis-driven filtration.

ABSTRACT: Extracellular vesicles (EVs) hold significant promise as biomarkers and therapeutics, yet their isolation remains challenging due to their low abundance and complex sample matrices. Here, we introduce EV-Osmoprocessor (EVOs), a novel device that leverages osmosis-driven filtration for rapid and efficient EV isolation. EVOs employs a high osmolarity polymer solution to concentrate EVs while simultaneously removing smaller contaminants. Compared to traditional methods such as ultracentrifugation and precipitation, EVOs offers speed and convenience, achieving a 50-fold volume reduction in under 2 h. Our results show that EVOs retained EVs and removed >99% albumin from the cell conditioned culture medium (CCM). The isolated EVs exhibited a particle size distribution centered around 140 nm, whi

SUBMITTER: Huang CY 

PROVIDER: S-EPMC12001845 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature