Dual genetic tracing demonstrates the heterogeneous differentiation and function of neuromesodermal progenitors in vivo.

ABSTRACT: In recent decades, the traditional paradigm of three distinct germ layers formed during gastrulation has been revised with the identification of neuromesodermal progenitors (NMPs). These progenitors emerge during gastrulation and contribute to both the neural ectoderm, particularly the spinal cord, and the adjacent paraxial mesoderm [D. Henrique et al., Development 142, 2864-2875 (2015); R. J. Garriock et al., Development 142, 1628-1638 (2015); E. Tzouanacou et al., Dev. Cell 17, 365-376 (2009)]. However, effective genetic tools for lineage tracing and functional assessments of NMPs in vivo are currently lacking. Here, we developed a dual recombinase-mediated genetic system to specifically trace and ablate Brachyury⁺

SUBMITTER: Jin H 

PROVIDER: S-EPMC12002027 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature