Lysosomal PIP₃ revealed by genetically encoded lipid biosensors.

ABSTRACT: 3-Phosphoinositides (3-PIs), phosphatidylinositol (3,4) bisphosphate [PI(3,4)P₂] and phosphatidylinositol (3,4,5) trisphosphate (PIP₃), are important lipid second messengers in the Phosphoinositide 3-Kinase (PI3K)/Akt signaling pathway, which is crucial to cell growth and frequently dysregulated in cancer. Emerging evidence suggests these lipid second messengers may be present in membranes beyond the plasma membrane, yet their spatial regulation within other membrane compartments is not well understood. To dissect the spatial regulation of specific 3-PI species, we developed genetically encodable biosensors with selectivity for PIP₃ or PI(3,4)P₂. Using these biosensors, we showed that PIP₃ significantly accumulated at the lysosome upon

SUBMITTER: Sahan AZ 

PROVIDER: S-EPMC12002240 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature