HEATR3 recognizes membrane rupture and facilitates xenophagy in response to <i>Salmonella</i> invasion.

ABSTRACT: Bacterial invasion into the cytoplasm of epithelial cells triggers the activation of the cellular autophagic machinery as a defense mechanism, a process known as xenophagy. In this study, we identified HEATR3, an LC3-interacting region (LIR)-containing protein, as a factor involved in this defense mechanism using quantitative mass spectrometry analysis. HEATR3 localizes intracellularly invading Salmonella, and HEATR3 deficiency promotes Salmonella proliferation in the cytoplasm. HEATR3 also localizes to lysosomes damaged by chemical treatment, suggesting that Salmonella recognition is facilitated by damage to the host cell membrane. HEATR3 deficiency impairs LC3 recruitment to damaged membranes and blocks the delivery of the target to the lysosome. These phenotypes wer

SUBMITTER: Arakawa M 

PROVIDER: S-EPMC12002282 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature