Establishment of a simple prediction method for DNA melting temperature: high-resolution melting curve analysis of PCR products.

ABSTRACT: High-resolution melting analysis is a technique that leverages the principle that the thermal stability of dsDNA is influenced by its length and base composition. This method generates a melting curve by real-time monitoring of the changes in fluorescence signal as dsDNA melts during the heating process. The melting temperature serves as a fundamental indicator of sample characteristics in HRM analysis. During the initial stages of designing a new HRM experimental system, accurately predicting the Tm position of the established system can significantly enhance research efficiency. Currently, there is a limited number of studies focused on the prediction of Tm values in HRM analysis, with varying levels of predictive accuracy. The nearest-neighbor method model can well reflect the interacti

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC12002481 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature