Establishment of an I-ELISA method based on multi-epitope fusion protein for diagnosis of human brucellosis.

ABSTRACT:

Background

Brucellosis is a significant zoonotic disease that impacts people globally, and its diagnosis has long posed challenges. This study aimed to explore the application value of multi-epitope fusion protein in the diagnosis of human brucellosis.

Methods

Eight important Brucella outer membrane proteins (OMPs) were selected: BP26, omp10, omp16, omp25, omp2a, omp2b, and omp31. Bioinformatics techniques were used to predict the immune epitopes of these proteins, and a multi-epitope fusion protein was designed. This fusion protein was used as the antigen for indirect enzyme-linked immunosorbent assay (iELISA) testing on 100 positive and 96 negative serum samples. The performance of the fusion protein in diagnosing brucellosis was evaluated using receiver operating characte

SUBMITTER: Xie Y 

PROVIDER: S-EPMC12002633 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature