Enhanced chlorogenic acid production from glucose via systematic metabolic engineering of <i>Saccharomyces cerevisiae</i>.

ABSTRACT: Chlorogenic acid (CGA) is a valuable phenolic acid with various pharmaceutical functions. In our previous study, de novo synthesis of CGA in Saccharomyces cerevisiae was achieved. However, its yield required improvement before large scale production. In this study, systematic metabolic engineering strategy was used to reconstruct chassis cell S. cerevisiae YC0707 to enhance its CGA yield from glucose. To balance the supply of phosphoenolpyruvate (PEP) and erythrose 4-phosphate (E4P), ZWF1 (encoding glucose-6-phosphate dehydrogenase) and GND1 (encoding 6-phosphogluconate dehydrogenase) were overexpressed by strong promoter P _TEF1 swapping, thereby strengthening the pentose phosphate pathway. The mutant of phosphofructokinase

SUBMITTER: Tu S 

PROVIDER: S-EPMC12002710 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature