Combined metabolic and enzymatic engineering for <i>de novo</i> biosynthesis of δ-tocotrienol in <i>Yarrowia lipolytica</i>.

ABSTRACT: δ-Tocotrienol, an isomer of vitamin E with anti-inflammatory, neuroprotective and anti-coronary arteriosclerosis properties, is widely used in health care, medicine and other fields. Microbial synthesis of δ-tocotrienol offers significant advantages over plant extraction and chemical synthesis methods, including increased efficiency, cost-effectiveness and environmental sustainability. However, limited precursor availability and low catalytic efficiency of key enzymes remain major bottlenecks in the biosynthesis of δ-tocotrienol. In this study, we assembled the complete δ-tocotrienol biosynthetic pathway in Yarrowia lipolytica and enhanced the precursor supply, resulting in a titre of 102.8 mg/L. The catalytic efficiency of the rate-limiting steps in the pathway was then enhanced th

SUBMITTER: Xiang J 

PROVIDER: S-EPMC12002712 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature