Identification of two new flavone 4'-<i>O</i>-methyltransferases and their application in <i>de novo</i> biosynthesis of (<i>2S</i>)-hesperetin in <i>Yarrowia lipolytica</i>.

ABSTRACT: Methyltransferases are pivotal enzymes in the biosynthesis of methylated flavonoids, including (2S)-hesperetin. However, existing flavonoid 4'-O-methyltransferase (F4'OMT) enzymes typically exhibit low substrate specificity and catalytic efficiency, which hinders microbial synthesis. To overcome this limitation, this study screened and identified two novel F4'OMTs, CrcOMT-2 and CgtOMT-3, from Chinese citrus varieties Citrus reticulata 'Chachiensis' (CZG) and Citrus grandis Tomentosa (HZY). These enzymes displayed high substrate specificity for (2S)-eriodictyol. A strain capable of de novo synthesis of (2S)-hesperetin was developed by integrating the novel F4'OMTs and other biosynthetic pathway genes at high copy numbers into

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12002713 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature