The landscape of RNA 5-methylcytosine modification during chicken embryonic myogenesis.

ABSTRACT: Skeletal muscle is a vital protein source for human diets, making its development a significant focus in poultry research. This study examines the effects of RNA 5-methylcytosine (m5C) modifications on chicken skeletal muscle development at two critical embryonic stages: before myoblast differentiation (E10) and after differentiation (E19). By employing MeRIP-seq, we analyzed the dynamic distribution of m5C modifications within the leg muscle transcriptome, uncovering notable differences in modification states between these two stages. Our results indicate that m5C modifications are widespread in chicken skeletal muscle transcriptome and present a unique distribution pattern. Unlike N6-methyladenosine modifications, which typically show a negative correlation with RNA expression, m5C modif

SUBMITTER: Jiang H 

PROVIDER: S-EPMC12002773 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature