Choice of numerical implementation of spatial contrast calculation impacts microcirculation quantitation in laser speckle contrast imaging.

ABSTRACT:

Significance

Laser speckle contrast imaging (LSCI) allows noninvasive imaging of microcirculation. Its scope of clinical applications is growing, yet the literature lacks a comparison of the accuracy of methods used to compute the spatial contrast Ks from which the blood flow index is derived.

Aim

We aim to evaluate the impact on flow quantitation of different computational approaches used to derive Ks .

Approach

We compare numerical calculation of Ks in Python and ImageJ applied to noise-free simulated data and to experimental data acquired in vivo in anesthetized mice. The estimation of the decorrelation time τc , inversely proportional to the blood flow index, is carried out following two approaches: LSCI asymptotic estimation and fitting the multiple exposu

SUBMITTER: Chammas M 

PROVIDER: S-EPMC12003051 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature