Metagenomic analysis reveals the diversity of the vaginal virome and its association with vaginitis.

ABSTRACT:

Introduction

The human vaginal virome is an essential yet understudied component of the vaginal microbiome. Its diversity and potential contributions to health and disease, particularly vaginitis, remain poorly understood.

Methods

We conducted metagenomic sequencing on 24 pooled vaginal swab libraries collected from 267 women, including both healthy individuals and those diagnosed with vaginitis. Viral community composition, diversity indices (Shannon, Richness, and Pielou), and phylogenetic characteristics were analyzed. Virus-host associations were also investigated.

Results

DNA viruses dominated the vaginal virome. Anelloviridae and Papillomaviridae were the most prevalent eukaryotic viruses, while Siphoviridae and Microviridae were the

SUBMITTER: Lu X 

PROVIDER: S-EPMC12003417 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature