Development of gene-in-plasmid DNA reference materials certified by single-molecule counting.

ABSTRACT: The mole, the SI unit for measuring the amount of a substance, was redefined as a fixed number of entities. This definition enables straightforward quantification of substances by counting individual entities. Counting proves particularly effective for quantifying large and discrete biological entities such as DNA, proteins, viruses, and cells, which are challenging to quantify via traditional physical or chemical methods. In this study, we detail our approach to develop gene reference materials certified through single-molecule counting, which enables mole-traceable measurements. We quantified three plasmid DNA constructs, each carrying a specific gene of interest, via single-molecule counting. The resulting values were cross-validated via digital PCR and LC‒MS. Sequence impurities in the

SUBMITTER: Lee DH 

PROVIDER: S-EPMC12003533 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature