RHOB regulates megakaryocytic and erythroid differentiation by altering the cell cycle and cytoskeleton.

ABSTRACT: RHOB, a member of the RHO GTPase family, is a target of miR-1915-3p. miR-1915-3p was proven to promote the differentiation of megakaryocytes while inhibiting that of the erythroid lineage. Although RHOB has been shown to be involved in proplatelet production in mice, its role in early haematopoiesis, especially megakaryocytic and erythroid differentiation in humans, has not yet been elucidated. In this study, we demonstrated the function of RHOB by knocking down the gene in progenitor cells and inducing these cells to differentiate into erythroid or megakaryocytic cells. The silencing of RHOB significantly decreased the proportion of erythroid cells and inhibited the expression of related genes during erythroid differentiation while increasing the proportion of megakaryocytic and polyploid

SUBMITTER: Liu P 

PROVIDER: S-EPMC12003728 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature