Single-cell chromatin landscapes associated with the burnt skin healing process in rats.

ABSTRACT: Thermal injuries represent one of the most severe forms of trauma to the human body, with a high annual incidence of burn victims globally. Skin regeneration and wound healing following thermal injury constitute a complex process involving various cell types and cytokine interactions. By Single-cell ATAC sequencing (scATAC-seq), we elucidated the molecular mechanisms underlying the dermal regeneration and healing processes following thermal injury in a rat model. Tissue samples were harvested for sequencing at predetermined intervals (0 h, 12 h, 24 h, 3 d, 7 d, 11 d, 15 d, and 19 d post-injury), yielding 28,179 high-quality single cells. Our comprehensive analysis revealed 28 distinct cell populations throughout the regenerative process, encompassing various subsets of keratinocytes, fibro

SUBMITTER: Wang T 

PROVIDER: S-EPMC12003776 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature