Detection of the GH analogue somatrogon in doping control urine samples by means of LC-HRMS/MS.

ABSTRACT: Somatrogon is a synthetic growth hormone (GH) analog, whose misuse in sports is prohibited at all times. As analytical approaches complementary to existing immunological GH detection methods were considered beneficial for its detection in doping control serum samples, a sensitive and specific qualitative mass spectrometric assay was recently developed, comprehensively characterized, and successfully employed to analyze authentic in vivo study samples. Within this follow-up project, this approach based on affinity purification, tryptic digestion, and LC-HRMS/MS was modified by implementing an additional ultrafiltration step to allow for the extraction of the intact drug and/or its metabolites from urine. Method validation demonstrated its specificity and sensitivity (LOD: 1 ng/mL), and the

SUBMITTER: Walpurgis K 

PROVIDER: S-EPMC12003886 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature