Infrared Free-Electron Laser: A Versatile Molecular Cutter for Analyzing Solid-State Biomacromolecules.

ABSTRACT: Free-electron lasers that oscillate in the infrared (IR) range of 1000 (10 μm) to 4000 cm^-1 (2.5 μm) were applied to irradiate solid-phase polysaccharides and aromatic biomacromolecules. Synchrotron radiation IR microscopy (SR-IRM) and electrospray ionization mass spectroscopy (ESI-MS) analyses showed that N-acetyl glucosamine was isolated from the powdered exoskeleton of crayfish by irradiation at 1020 cm^-1 (9.8 μm), resonating with the C-O stretching mode (νC-O). Irradiation at 3448 cm^-1 (2.9 μm), which is resonant with the O-H stretching vibration (νO-H) of sulfonated lignin, dissociates the aggregate state and releases coniferyl aldehyde substituted with sulfinate, as shown by scanning electron microscopy, terahertz-coherent edge radiation spectr

SUBMITTER: Kawasaki T 

PROVIDER: S-EPMC12004168 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature