Analysis of <i>O-G</i>lycans by Oxidative Release Combined with 3-Nitrophenylhydrazine Derivatization.

ABSTRACT: Glycosylation profiling is an effective methodology for achieving a comprehensive understanding of glycoproteins and their alterations in a multitude of pathological conditions. However, in comparison to N-glycosylation, O-glycosylation presents significant challenges in terms of both qualitative and quantitative mass spectrometric analyses. A recently developed oxidative release protocol enables the selective formation of O-glycans containing a carboxyl group derived from the amino acid residue. In this study, 3-nitrophenylhydrazine was used to derivatize the common carboxyl group in a mild hydrophilic solution. Derivatization resulted in the generation of a series of report ions for serine, threonine, sialic acid, and O-acetylated sialic acid residues, thereby

SUBMITTER: Min Z 

PROVIDER: S-EPMC12004196 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature