Characterization of a rhodopsin-phosphodiesterase from Choanoeca flexa to be combined with rhodopsin-cyclases for bidirectional optogenetic cGMP control.

ABSTRACT: Rhodopsin phosphodiesterases (RhPDEs) were first discovered in the choanoflagellate Salpingoeca rosetta, but their physiological role remained unknown. Their light-dependent modulation was found to be low, limiting optogenetic application. However, recent in vivo studies in the choanoflagellate Choanoeca flexa revealed a strong linkage of RhPDE to the actomyosin-mediated contraction and colony sheet inversion and identified downstream cGMP effectors. Through screening various RhPDE variants from C. flexa, we identified four photomodulated PDEs of which C. flexa RhPDE1 (CfRhPDE1) revealed the highest cGMP affinity and the most pronounced light regulation with K_m values of 1.9 and 4.4 μM in light and darkness. By coexpressing CfRhPDE1 with the rhodopsin-guanylyl-cyclase from the f

SUBMITTER: Liem N 

PROVIDER: S-EPMC12004702 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature