Molecular features and diagnostic modeling of synovium- and IPFP-derived OA macrophages in the inflammatory microenvironment via scRNA-seq and machine learning.

ABSTRACT:

Background

Osteoarthritis (OA) is the leading cause of degenerative joint disease, with total joint replacement as the only definitive cure. However, no disease-modifying therapy is currently available. Inflammation and fibrosis in the infrapatellar fat pad (IPFP) contribute to OA onset and progression. However, the cellular composition and molecular mechanisms in the IPFP microenvironment remain unclear. This study investigates the functions of OA-macrophages and their clinical significance.

Methods

We analyzed single-cell RNA sequencing (scRNA-seq) data from normal and OA patients. Enrichment analysis revealed differences in biological pathways across cell types. Pseudotime and cell-cell communication analyses revealed the developmental trajectory and interactions of OA-ma

SUBMITTER: Lin C 

PROVIDER: S-EPMC12004787 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature