Charged multivesicular body protein 4C promotes the progression of cervical cancer through the HPV E6/miR‑543 axis.

ABSTRACT: Charged multivesicular body protein 4C (CHMP4C), as a subunit of endosomal sorting complex required for transport-III, is important for the abscission checkpoint in cell division, preventing premature cell division and genetic damage. The present study aimed to assess the role of CHMP4C in cervical cancer and the associated mechanisms. The levels of CHMP4C in normal and cervical cancer tissues were detected by immunohistochemistry. The MTT assay, apoptosis, wound-healing assay, and cell invasion assay were performed. Western blotting was performed to analyze the level of cancer-related proteins following CHMP4C downregulation and the CHMP4C expression following E6 downregulation and miR-543 upregulation. The transfection effectiveness of siRNA, plasmid, and miRNA mimic as well as the expre

SUBMITTER: Liu RC 

PROVIDER: S-EPMC12005071 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature