Phyloproteomics reveals conserved patterns of axonemal dynein methylation across the motile ciliated eukaryotes.

ABSTRACT: Axonemal dynein assembly occurs in the cytoplasm and numerous cytosolic factors are specifically required for this process. Recently, one factor (DNAAF3/PF22) was identified as a methyltransferase. Examination of Chlamydomonas dyneins found they are methylated at substoichiometric levels on multiple sites, including Lys and Arg residues in several of the nucleotide-binding domains and on the microtubule-binding region. Given the highly conserved nature of axonemal dyneins, one key question is whether methylation happens only in dyneins from the chlorophyte algae, or whether these modifications occur more broadly throughout the motile ciliated eukaryotes. Here we take a phyloproteomic approach and examine dynein methylation in a wide range of eukaryotic organisms bearing motile cilia

SUBMITTER: Sakato-Antoku M 

PROVIDER: S-EPMC12005109 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature