A targeted CRISPR screen identifies ETS1 as a regulator of HIV-1 latency.

ABSTRACT: Human Immunodeficiency virus (HIV) infection is regulated by a wide array of host cell factors that combine to influence viral transcription and latency. To understand the complex relationship between the host cell and HIV-1 latency, we performed a lentiviral CRISPR screen that targeted a set of host cell genes whose expression or activity correlates with HIV-1 expression. We further investigated one of the identified factors - the transcription factor ETS1, and found that it is required for maintenance of HIV-1 latency in both latently infected cell lines and in a primary CD4 T cell latency model. Interestingly, ETS1 played divergent roles in actively infected and latently infected CD4 T cells, with knockout of ETS1 leading to reduced HIV-1 expression in actively infected cells, but incre

SUBMITTER: Ashokkumar M 

PROVIDER: S-EPMC12005537 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature