Inhibitory Effect of Whey Protein-Derived Peptide Leu-Asp-Gln-Trp on Xanthine Oxidase.

ABSTRACT: Hyperuricemia is associated with various diseases, and xanthine oxidase (XO) is the rate-limiting enzyme in uric acid (UA) production. A previous study reported that Leu-Asp-Gln-Trp (LDQW) in whey protein hydrolysate (WPH) suppressed lipid droplet accumulation in differentiated 3T3-L1 adipocyte-like cells. However, our understanding of LDQW remains limited, further, its efficacy against hyperuricemia has not been elucidated. This study evaluated the XO inhibitory activity of LDQW, one of the bioactive peptides in WPH. In this study, UA produced by the reaction between XO and xanthine was determined using two methods: monitoring the absorbance at 290 nm using absorptiometry and detection using liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis. Allopurinol was used as t

SUBMITTER: Toda K 

PROVIDER: S-EPMC12006031 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature