Repertoire-based mapping and time-tracking of T helper cell subsets in scRNA-Seq.

ABSTRACT:

Introduction

The functional programs of CD4⁺ T helper (Th) cell clones play a central role in shaping immune responses to different challenges. While advances in single-cell RNA sequencing (scRNA-Seq) have significantly improved our understanding of the diversity of Th cells, the relationship between scRNA-Seq clusters and the traditionally characterized Th subsets remains ambiguous.

Methods

In this study, we introduce TCR-Track, a method leveraging immune repertoire data to map phenotypically sorted Th subsets onto scRNA-Seq profiles.

Results and discussion

This approach accurately positions the Th1, Th1-17, Th17, Th22, Th2a, Th2, T follicular helper (Tfh), and regulatory T-cell (Treg) subsets, outperforming mapping based on CITE-Seq. Remarkably, the mappi

SUBMITTER: Lukyanov DK 

PROVIDER: S-EPMC12006041 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature