GSDME knockout alleviates hematopoietic stem cell irradiation injury and aggravates myeloid-biased differentiation.

ABSTRACT:

Background

Bone marrow (BM) suppression is the most prevalent dose-limiting side effect of chemotherapy and radiotherapy. Exposure to ionizing radiation (IR) results in acute BM suppression and long-term BM injury. Gasdermin E (GSDME) is crucial for mediating apoptosis and pyroptosis during chemotherapy. However, its role in radiation-induced hematopoietic injury is not well established. Therefore, we aimed to investigate the role of GSDME on radiation-induced hematopoietic injury.

Methods

We established hematopoietic radiation injury models in C57BL/6 mice and Gsdme^-/- mice. The peripheral blood (PB) counts, phenotypes of BM cells and spleen cells were analyzed. The colony-forming unit-granulocyte and macrophage assays and competitive repopulation assays were mea

SUBMITTER: Su L 

PROVIDER: S-EPMC12006135 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature