In vitro antidiabetic and antioxidant activities of protein hydrolysates via alkaline autolysis of Fusarium venenatum mycoprotein.

ABSTRACT: The mycoprotein from Fusarium venenatum, characterized by its abundant proteins could be a valuable candidate for autolysis, potentially contributing in the production of bioactive compounds. In this study, four autolysis methods, including acidic, alkaline, plasmolysis, and enzymatic hydrolysis were applied to enhance the extraction of bioactive compounds. The resulting protein hydrolysates were evaluated for their antioxidant properties, α-amylase and α-glucosidase inhibition, antibacterial activity against Escherichia coli, Salmonella spp., and Staphylococcus aureus, antifungal activity against Aspergillus niger, and structural characteristics. Antioxidant assays revealed that alkaline autolysates exhibited the highest activity (DPPH: ~ 556 µmol Trolox/g sample; ABTS: ~ 235 µmol Trolox/

SUBMITTER: Davoudi M 

PROVIDER: S-EPMC12006368 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature