Doxorubicin delivery of green-synthesized gold nanoparticles to MDA-MB-231 cells and collagen type II identification from yellow nose skate cartilage.

ABSTRACT: Upcycling and green synthesis strategies have been applied for the synthesis of gold nanoparticles (AuNPs) using yellow nose skate (Dipturus chilensis) cartilage extract as a reducing and stabilizing agent (skAu). Doxorubin (DOX) was loaded onto the skAu, and the average size of the DOX-loaded AuNPs (c-skAuD) was measured to be 14.6 ± 0.5 nm by high-resolution transmission electron microscopy. Reaction parameters such as reaction time and DOX concentration were optimized for preparing c-skAuD. DOX was successfully loaded, as confirmed by the FT-IR results. In addition, FT-IR characterization revealed that chondroitin sulfate and collagen in the extract were involved in the synthesis of skAu. Although c-skAuD is cytotoxic to four different types of cancer cells (AGS, HeLa, A549 and MDA-MB-2

SUBMITTER: Ahn EY 

PROVIDER: S-EPMC12006596 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature