Uncovering a novel biosynthetic gene cluster for sordarin through genome mining in the fungus Talaromyces adpressus.

ABSTRACT: To explore the chemical and biological diversities of diterpenoids from the fungus Talaromyces adpressus, a previously unknown biosynthetic gene cluster (BGC, tdn) for sordarin (a well-known fungal antibiotics) was discovered by leveraging the genome mining method. Heterologous expressions of key genes of tdn in Aspergillus oryzae, led to the determination of one new diterpenoid, cycloaraneosene-9-ol-8-one (4), and three known diterpenoids, cycloaraneosene (1), cycloaraneosene-9-ol (2), cycloaraneosene-8,9-diol (3). The structures of 1-4 was elucidated well via detailed analysis of 1D and 2D NMR, GCMS, HRESIMS, IR data, and comparison with reported data. Structurally, compounds 1-4 were belonging to fusicoccane diterpenoids with a classical tricyclic 5/8/5 ring system, which are participat

SUBMITTER: Xu Q 

PROVIDER: S-EPMC12006653 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature