Expedient Assembly of Multiantennary <i>N</i>-Glycans from Common <i>N</i>-Glycan Cores with Orthogonal Protection for the Profiling of Glycan-Binding Proteins.

ABSTRACT: Complex-type N-glycans are structurally diverse molecules, responsible for many biological processes, yet the specific sequences of N-glycans involved in biological recognition remain largely unknown. Despite the recent development of many efficient chemoenzymatic approaches, it is still lacking a general approach to produce structurally diverse complex-type N-glycans. Here, we designed two common precursors equipped with orthogonal protecting groups for antennary differentiation and selective glycan elongation. The N-acetyllactosamine (LacNAc) repeat modules were synthesized separately based on iterative Au(I) promoted glycosylation and programmable one-pot strategy and were incorporated into the N-glycan core structure in a site-specific manner. The fin

SUBMITTER: Li R 

PROVIDER: S-EPMC12006998 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature